Quantitative Determination of Amitriptyline by GC-FID Using Derivatization Method

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Nidhi Tangade
Mukesh Mohite
Sonali Mahaparale
Tejas Dhanuka
Shruti Nilakh
Niharika Mahale


A novel and new gas chromatographic technique has been developed for the quantitative determination of Amitriptyline in bulk drug as well as pharmaceutical dosage form. Ethyl acetate was used as a precolumn derivatizing agent. The separation by GC was carried out by using 10% OV-17 column (bonded and crosslinked 50% diphenyl 50% dimethylpolysiloxane) in combination with flame ionization detector (FID). Nitrogen was used as carrier gas. For the elution, the initial temperature of oven was maintained at 1000 C for 1 min. and then the temperature was increased at the rate of 100 C per min. The temperature of detector and sample injector were kept isothermal at 2000 C. Various parameters were used for the validation of developed method. The calibration curve was found to be linear in the concentration range of 1-10 µg/ml. and correlation coefficient (r2) value was found to be 0.9984. The % assay was found to be 99.32% with standard deviation of 0.0402 and % RSD value of 0.4050. The developed method is highly precise, specific, accurate and reproducible and could be applied for derivatization and estimation of amitriptyline in its pure and pharmaceutical dosage form.    

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Tangade, N. ., Mohite, M. ., Mahaparale, S. ., Dhanuka, T. ., Nilakh, S. ., & Mahale, N. . (2023). Quantitative Determination of Amitriptyline by GC-FID Using Derivatization Method. Journal of Coastal Life Medicine, 11(2), 200–205. Retrieved from https://jclmm.com/index.php/journal/article/view/942


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