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The present investigation aimed to evaluate effect of xanthotoxin on potentiating anticonvulsant activity of Rauwolfia serpentina on the chemically induced convulsions in experimental animals and compare the effect of xanthotoxin on anticonvulsant activity of Rauwolfia serpentina with the standard dose of Diazepam on Pentylenetetrazol and Strychnine HCL model. Moreover, brain GABA levels were estimated to investigate the mechanisms underlying the anticonvulsant activity. In this study, results indicate that xanthotoxin (50 and 100 mg/kg, i.p.) significantly potentiated anticonvulsant activity of Rauwolfia serpentina. Similarly, xanthotoxin (100 mg/kg, i.p.) markedly enhanced the anticonvulsant action of Rauwolfia serpentina showed significant and dose-dependent inhibition of PTZ and Strychnine-induced convulsions. Furthermore, addition of xanthotoxin with Rauwolfia serpentina showed significantly increased GABA levels in brain as compared to Rauwolfia serpentina. The parameters observed in PTZ and Strychnine-induced models were seizure latency, duration, and mortality. Combination of xanthotoxin with Rauwolfia serpentina showed significant anticonvulsant activity in experimental animals.
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